4II9
Crystal structure of Weissella viridescens FemXVv non-ribosomal amino acid transferase in complex with a peptidyl-RNA conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.130, 101.770, 46.320 |
| Unit cell angles | 90.00, 102.60, 90.00 |
Refinement procedure
| Resolution | 38.120 - 1.660 |
| R-factor | 0.17661 |
| Rwork | 0.175 |
| R-free | 0.20935 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gkr |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.342 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.120 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.040 | 0.189 |
| Number of reflections | 44718 | |
| <I/σ(I)> | 23.8 | 6.9 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 0.1M Tris-sodium citrate buffer, 0.2M ammonium acetate, 33% polyethylene glycol 4k, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
