4IHL
Human 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide and Cotylenin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9778 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.280, 103.400, 112.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.200 |
| R-factor | 0.2024 |
| Rwork | 0.200 |
| R-free | 0.24320 |
| Structure solution method | MIR |
| Starting model (for MR) | 4fj3 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.575 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.300 | |
| High resolution limit [Å] | 2.200 | 12.000 | 2.200 |
| Rmerge | 0.035 | 0.019 | 0.280 |
| Number of reflections | 42870 | 318 | 5204 |
| <I/σ(I)> | 21.65 | 53.62 | 4.66 |
| Completeness [%] | 98.3 | 95.5 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M NA-ACETATE PH 7.0, 0.8 M NAH2PO4 AND 1.2 M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
