4IG7

Crystal structure of Trichinella spiralis UCH37 bound to Ubiquitin vinyl methyl ester

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-10-07
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.033
Spacegroup name	H 3
Unit cell lengths	147.361, 147.361, 40.485
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.590 - 1.996
R-factor	0.1954
Rwork	0.193
R-free	0.23950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i6n
RMSD bond length	0.008
RMSD bond angle	1.143
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.590	2.030
High resolution limit [Å]	1.996	2.000
Rmerge	0.085
Number of reflections	22270
<I/σ(I)>	4.9
Completeness [%]	100.0	100
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	293	0.2 M ammonium chloride, 18% PEG3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K