4IFN
Crystal Structures of apo Keap1, Keap1-peptide, and Keap1-compound complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 180 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-08-30 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.878, 75.258, 48.754 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 35.280 - 2.400 |
| R-factor | 0.27847 |
| Rwork | 0.274 |
| R-free | 0.35244 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.828 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.280 | 2.462 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 15676 | |
| Completeness [%] | 96.7 | 99.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.1 | 277 | 0.6 M Sodium Formate, 0.2 M Ammonium Acetate, 0.1 M Tris pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
