4IEM
Human apurinic/apyrimidinic endonuclease (APE1) with product DNA and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Collection date | 2001-07 |
Wavelength(s) | 0.97956 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.639, 74.090, 112.138 |
Unit cell angles | 90.00, 111.98, 90.00 |
Refinement procedure
Resolution | 29.643 - 2.394 |
R-factor | 0.2099 |
Rwork | 0.208 |
R-free | 0.24620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 0.918 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.393 |
Number of reflections | 56727 |
Completeness [%] | 90.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 50 mM MES pH 6.0, 200 mM LiSO4, and 25% mPEG 2K, vapor diffusion, hanging drop, temperature 298K |