4I7H

Structural basis for peroxide sensing and gene regulation by PerR from Streptococcus pyogenes

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796, 0.957, 0.9798
Spacegroup name	P 63 2 2
Unit cell lengths	118.910, 118.910, 118.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.231 - 2.000
R-factor	0.2312
Rwork	0.229
R-free	0.26450
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.125
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	33859
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	2 M sodium chloride, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	8	298	2 M sodium chloride, 0.1 M Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K