4I6L

Crystal structure of OTUB1 in complex with ubiquitin variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	93
Detector technology	PIXEL
Collection date	2012-07-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	92.444, 109.069, 73.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.222 - 2.488
R-factor	0.2218
Rwork	0.220
R-free	0.26630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zfy 1ubq
RMSD bond length	0.003
RMSD bond angle	0.715
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.500	2.590
Number of reflections	12681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M ammonium sulfate, 0.1 M MES, pH 6.5, and 20% (w/v) PEG8000, VAPOR DIFFUSION, SITTING DROP, temperature 293K