4I60
Crystal structure of avidin - biotinylruthenocene complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8015 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 60.220, 60.220, 62.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.500 |
| R-factor | 0.16711 |
| Rwork | 0.165 |
| R-free | 0.21103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vyo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.264 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.360 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.132 | 0.505 |
| Number of reflections | 4301 | |
| <I/σ(I)> | 19.49 | 4.75 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 9.07 | 8.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 35% pentaerythritol propoxylate, 0.05M HEPES, 0.2M KCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
