4I27
Ternary complex of mouse TdT with ssDNA and incoming nucleotide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.940, 95.900, 47.230 |
| Unit cell angles | 90.00, 107.75, 90.00 |
Refinement procedure
| Resolution | 47.950 - 2.600 |
| R-factor | 0.1741 |
| Rwork | 0.171 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jms |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.950 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.137 | 0.461 |
| Number of reflections | 12246 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG4000, 20% isopropanol, 100 mM sodium citrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
