4HYO
Crystal Structure of MthK Pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.035, 63.452, 63.477 |
| Unit cell angles | 90.03, 89.99, 89.99 |
Refinement procedure
| Resolution | 44.864 - 1.650 |
| R-factor | 0.1646 |
| Rwork | 0.164 |
| R-free | 0.18090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 3.550 | 1.650 |
| Rmerge | 0.042 | 0.027 | 0.397 |
| Number of reflections | 79424 | ||
| <I/σ(I)> | 14.5 | ||
| Completeness [%] | 96.3 | 99.1 | 93.8 |
| Redundancy | 3.2 | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3.5-4.0M 1,6-hexanediol, 100mM MES , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
