4HVX
tRNA-guanine transglycosylase Y106F, C158V mutant in complex with queuine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 113.15 |
| Detector technology | CCD |
| Collection date | 2010-12-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.662, 64.486, 71.125 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 23.824 - 1.820 |
| R-factor | 0.1691 |
| Rwork | 0.166 |
| R-free | 0.19750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3GEV |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.158 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 35405 | |
| <I/σ(I)> | 21.7 | 2.2 |
| Completeness [%] | 94.9 | 78.4 |
| Redundancy | 3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 100 mM Tris, 1 mM DTT, 10% DMSO, 12 % PEG 8000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K |
