4HTI
Crystallographic structure of the membrane-proximal ectodomain of the human receptor-type protein-tyrosine phosphatase phogrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91840 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 55.133, 55.133, 148.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.290 - 1.954 |
| R-factor | 0.2223 |
| Rwork | 0.221 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qt7 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.563 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.280 | 1.950 |
| Rmerge | 0.060 | 0.032 | 0.993 |
| Number of reflections | 10352 | ||
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.6 | 94.6 | 100 |
| Redundancy | 40.5 | 32.6 | 41.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.2 M Ammonium sulfate, 30 % w/v Polyethylene glycol 4000, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
