4HQJ

Crystal structure of Na+,K+-ATPase in the Na+-bound state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-19
Detector	PSI PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	109.073, 219.578, 261.958
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	11.999 - 4.300
R-factor	0.2626
Rwork	0.261
R-free	0.28780
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	1.125
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000
High resolution limit [Å]	4.300	4.300
Number of reflections	43585
Completeness [%]	99.9	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	278	19% PEG2KMME, 150mM NaCl, 6% glycerol, 6% MPD, 25mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K
1	VAPOR DIFFUSION, HANGING DROP	7.5	278	19% PEG2KMME, 150mM NaCl, 6% glycerol, 6% MPD, 25mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 278K