4HLY
The complex crystal structure of the DNA binding domain of vIRF-1 from the oncogenic KSHV with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-09-29 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.96722 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.536, 38.577, 48.562 |
| Unit cell angles | 91.32, 88.48, 63.13 |
Refinement procedure
| Resolution | 19.530 - 1.480 |
| R-factor | 0.19792 |
| Rwork | 0.196 |
| R-free | 0.22788 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.107 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.106 | |
| Number of reflections | 41661 | |
| <I/σ(I)> | 2.3 | |
| Completeness [%] | 99.5 | 92.8 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.1M phosphate citrate pH 4.2, 40% PEG 300, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
