4HI9
1.2 structure of integrin-linked kinase ankyrin repeat domain in complex with PINCH1 LIM1 domain collected at wavelength 0.91974
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.91974 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.144, 41.775, 46.798 |
| Unit cell angles | 78.67, 68.94, 85.81 |
Refinement procedure
| Resolution | 34.662 - 1.203 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.19820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f6q |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.061 | 0.990 |
| Number of reflections | 75043 | |
| <I/σ(I)> | 15.685 | 1.327 |
| Completeness [%] | 94.2 | 90.9 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 20% PEG 3350, 0.2M Sodium Formate, 0.5M Sodium Iodide, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
