4HG3
Structural insights into yeast Nit2: wild-type yeast Nit2 in complex with alpha-ketoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97907 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.211, 125.653, 77.964 |
| Unit cell angles | 90.00, 95.30, 90.00 |
Refinement procedure
| Resolution | 48.840 - 1.930 |
| R-factor | 0.19833 |
| Rwork | 0.197 |
| R-free | 0.22675 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h5u |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.006 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.687 | |
| Number of reflections | 92897 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 287 | 17.5% PEG4000, 0.1M sodium cacodylate, 25mM 2-oxoglutaric acid sodium salt, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
