4HFH
The GLIC pentameric Ligand-Gated Ion Channel (wild-type) complexed to bromoform
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2011-11-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9191 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 181.000, 132.650, 159.270 |
Unit cell angles | 90.00, 102.16, 90.00 |
Refinement procedure
Resolution | 21.710 - 2.650 |
R-factor | 0.2025 |
Rwork | 0.202 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | BUSTER (2.11.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 2.790 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.082 | 0.821 |
Number of reflections | 105994 | |
<I/σ(I)> | 15 | 2.1 |
Completeness [%] | 99.1 | 96.1 |
Redundancy | 8.8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate, 0.4M NaSCN, 2% bromoform, VAPOR DIFFUSION, HANGING DROP, temperature 291K |