4HFB
The GLIC pentameric Ligand-Gated Ion Channel F14'A ethanol-sensitive mutant (Apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 183.737, 133.512, 161.120 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.750 |
| R-factor | 0.2085 |
| Rwork | 0.208 |
| R-free | 0.22230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.600 | 48.630 | 2.900 |
| High resolution limit [Å] | 2.750 | 8.690 | 2.750 |
| Rmerge | 0.044 | 0.018 | 0.630 |
| Number of reflections | 97187 | ||
| <I/σ(I)> | 15.6 | 50.6 | 2 |
| Completeness [%] | 98.8 | 97.3 | 96.7 |
| Redundancy | 3.4 | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 12-15% PEG4K, 0.1M Na Acetate 0.4M NaSCN, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
