4HBC

Crystal structure of a conformation-dependent rabbit IgG Fab specific for amyloid prefibrillar oligomers

Replaces:  3NL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979462
Spacegroup name	C 1 2 1
Unit cell lengths	132.934, 42.184, 86.179
Unit cell angles	90.00, 114.93, 90.00

Refinement procedure

Resolution	31.000 - 1.540
R-factor	0.2004
Rwork	0.197
R-free	0.25920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.507
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	PHASER (1.3.1)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	31.010	31.010	1.600
High resolution limit [Å]	1.540	3.320	1.540
Rmerge	0.081	0.049	0.530
Total number of observations		43516	14927
Number of reflections	56536
<I/σ(I)>	10.2	25.4	2
Completeness [%]	87.6	95.9	49.7
Redundancy	6.46	6.7	4.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Magnesium sulfate 20 % PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K