4H8I
Structure of GluK2-LBD in complex with GluAzo
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-28 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 102.609, 102.609, 282.513 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.920 - 2.000 |
R-factor | 0.175 |
Rwork | 0.173 |
R-free | 0.20390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g3f |
RMSD bond length | 0.017 |
RMSD bond angle | 1.573 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.100 |
High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
Rmerge | 0.070 | 0.014 | 0.605 |
Number of reflections | 60248 | 512 | 8042 |
<I/σ(I)> | 27.54 | 94.98 | 3.98 |
Completeness [%] | 99.8 | 84.5 | 100 |
Redundancy | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.05 M lithium sulfate, 10 mM magnesium chloride, 50 mM MES sodium, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |