4H2F
Human ecto-5'-nucleotidase (CD73): crystal form I (open) in complex with adenosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-09 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 167.548, 167.548, 167.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.890 - 1.850 |
R-factor | 0.15754 |
Rwork | 0.157 |
R-free | 0.17929 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 2.378 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.890 |
High resolution limit [Å] | 1.850 |
Number of reflections | 68775 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 292 | 1.0 M lithium chloride, 0.1 M Hepes , pH 7.0, VAPOR DIFFUSION, temperature 292K |