4H1S

Crystal Structure of a Truncated Soluble form of Human CD73 with Ecto-5'-Nucleotidase activity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9173
Spacegroup name	P 2 21 21
Unit cell lengths	54.910, 95.010, 230.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.860 - 2.200
R-factor	0.1762
Rwork	0.175
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.131
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.860	2.330
High resolution limit [Å]	2.200	2.200
Number of reflections	62077
Completeness [%]		97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	SPG buffer 0.1M pH4.0, 15% Peg 1500, drop volumes 200nl plus 200nl, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K