4GZL
Crystal structure of RAC1 Q61L mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.830, 72.940, 91.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.18937 |
| Rwork | 0.186 |
| R-free | 0.25021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3th5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.625 |
| Number of reflections | 23219 | |
| <I/σ(I)> | 14.244 | 3.541 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 296 | 16-18% PEG4000, 6% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
