4GZ3
Crystal structure of human O-GlcNAc Transferase with UDP and a thioglycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 98.770, 137.620, 153.016 |
| Unit cell angles | 90.00, 102.82, 90.00 |
Refinement procedure
| Resolution | 48.154 - 1.900 |
| R-factor | 0.2274 |
| Rwork | 0.226 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.7.2_869) |
| Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.870 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 154828 | |
| Completeness [%] | 99.1 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M Lithium Sulfate, 0.1M Bis Tris Propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
