4GZ2
Mus Musculus Tdp2 excluded ssDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.483, 42.972, 107.951 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 24.472 - 1.850 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.24540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.119 | 0.084 | 0.404 |
| Number of reflections | 45439 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 97.4 | 97.5 | 93.6 |
| Redundancy | 3.1 | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 22% PEG 3350, 100mM Tris, 250mM Mg(CO2)2, pH 8.5 , VAPOR DIFFUSION, temperature 293K |
