4GYZ
Mus Musculus Tdp2 Bound to dAMP and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-22 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.347, 169.326, 184.959 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.977 - 2.556 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.22020 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.640 |
High resolution limit [Å] | 2.550 | 5.490 | 2.550 |
Rmerge | 0.105 | 0.064 | 0.510 |
Number of reflections | 82947 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 97.7 | 97.7 | 92.1 |
Redundancy | 5.3 | 5.8 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | PEG 6000, 10mM dAMP, 100mM MES, 10mM MgCl2, pH 6.0, VAPOR DIFFUSION, temperature 293K |