4GYW

Crystal structure of human O-GlcNAc Transferase in complex with UDP and a glycopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-29
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	I 1 2 1
Unit cell lengths	99.000, 137.770, 153.566
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	43.904 - 1.700
R-factor	0.1881
Rwork	0.187
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.972
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.904	1.790
High resolution limit [Å]	1.700	1.700
Number of reflections	206769
Completeness [%]	94.3	87.1
Redundancy	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	1.6M Lithium Sulfate, 0.1M Bis Tris Propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K