4GYW
Crystal structure of human O-GlcNAc Transferase in complex with UDP and a glycopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 99.000, 137.770, 153.566 |
| Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
| Resolution | 43.904 - 1.700 |
| R-factor | 0.1881 |
| Rwork | 0.187 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.972 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX (1.7_650) |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.904 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 206769 | |
| Completeness [%] | 94.3 | 87.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.6M Lithium Sulfate, 0.1M Bis Tris Propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
