4GYB
Two-domain laccase from streptomyces lividans at 2.4 A resolution AC1709
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-08 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 43 3 2 |
Unit cell lengths | 177.540, 177.540, 177.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.385 - 2.400 |
R-factor | 0.1548 |
Rwork | 0.154 |
R-free | 0.17710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kw8 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.119 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 37874 | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 8.68 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 296 | 31%(v/v) PEG monomethyl ether 550, 0.1M NaCl, 0.1M glycine, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 296K |