4GXX
Crystal structure of the "avianized" 1918 influenza virus hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2012-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.790, 241.510, 72.030 |
| Unit cell angles | 90.00, 119.77, 90.00 |
Refinement procedure
| Resolution | 35.895 - 1.799 |
| R-factor | 0.1817 |
| Rwork | 0.180 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ruz |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.266 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.799 | 1.799 |
| Number of reflections | 193879 | |
| <I/σ(I)> | 15.9 | 2.2 |
| Completeness [%] | 98.5 | 92.4 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 100 mM Tris, pH 8.0, 40% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
