4GXU
Crystal structure of antibody 1F1 bound to the 1918 influenza hemagglutinin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2009-01-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 153.711, 176.269, 168.120 |
| Unit cell angles | 90.00, 92.14, 90.00 |
Refinement procedure
| Resolution | 35.036 - 3.294 |
| R-factor | 0.2206 |
| Rwork | 0.219 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gxv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.245 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.294 | 3.294 |
| Number of reflections | 108722 | |
| <I/σ(I)> | 14.2 | 1.9 |
| Completeness [%] | 80.6 | 34.9 |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.9 | 293 | 8.5% PEG6000, 100 mM Tris, pH 7.9, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
