4GXS
Ligand binding domain of GluA2 (AMPA/glutamate receptor) bound to (-)-kaitocephalin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.741, 121.466, 48.899 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.388 - 1.963 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.22460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.963 | 1.963 |
| Rmerge | 0.086 | 0.622 |
| Number of reflections | 41806 | |
| <I/σ(I)> | 14.267 | 4.118 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 16-18% PEG 8K, 0.1 M sodium cacodylate, 0.1-0.15 M zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
