4GV9
Lassa nucleoprotein C-terminal domain in complex with triphosphated dsRNA soaking for 5 min
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.780, 44.780, 221.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.890 - 2.460 |
| R-factor | 0.261 |
| Rwork | 0.259 |
| R-free | 0.30466 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.911 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.900 | 2.600 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.058 | 0.785 |
| Number of reflections | 8461 | |
| <I/σ(I)> | 2.4 | 5.6 |
| Completeness [%] | 98.4 | 99 |
| Redundancy | 5.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 18.5% PEG MME 5000, 0.1 M sodium citrate (pH4.5), and 2.4% PEG MME350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
