4GV3
Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.580, 46.580, 208.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.210 - 1.680 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22400 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.016 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.240 | 1.770 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmerge | 0.068 | 0.449 |
| Number of reflections | 25994 | |
| <I/σ(I)> | 9.1 | 2.6 |
| Completeness [%] | 100.0 | 96.1 |
| Redundancy | 3.2 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 18.5% PEGMME 5000, 0.1M sodium citrate pH4.5 and 2.4% PEGMME350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
