4GUU

Crystal structure of LSD2-NPAC with tranylcypromine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97906
Spacegroup name	P 1 21 1
Unit cell lengths	62.820, 90.157, 87.824
Unit cell angles	90.00, 104.20, 90.00

Refinement procedure

Resolution	36.232 - 2.302
R-factor	0.2004
Rwork	0.199
R-free	0.23130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gu1
RMSD bond length	0.004
RMSD bond angle	0.772
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_353))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	37948
Completeness [%]	90.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.02M Citric acid, 0.03M Bis_tris propane, 10% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K