4GUK
New crystal form structure of human NCS1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 31 |
Unit cell lengths | 54.987, 54.987, 213.954 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.750 |
R-factor | 0.2014 |
Rwork | 0.201 |
R-free | 0.24260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | human NCS1 |
RMSD bond length | 1.800 |
RMSD bond angle | 0.019 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.750 |
Rmerge | 0.091 |
Number of reflections | 73721 |
<I/σ(I)> | 11 |
Completeness [%] | 97.9 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | pH6.5, 0.2M NaAC, 30%PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |