4GUA
Alphavirus P23pro-zbd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2824 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 147.180, 147.180, 360.440 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.176 - 2.854 |
| R-factor | 0.1879 |
| Rwork | 0.186 |
| R-free | 0.21730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2HWK 3gpg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.109 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1066)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.050 | 3.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 1.103 | |
| Number of reflections | 105713 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 2 M ammonium sulfate, 0.1 M 2-(N-morpholino)ethane sulfonic acid (MES) (pH 6.5), VAPOR DIFFUSION, HANGING DROP, temperature 298K |
