4GTU
LIGAND-FREE HOMODIMERIC HUMAN GLUTATHIONE S-TRANSFERASE M4-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-08 |
| Detector | SIEMENS X1000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.000, 215.700, 94.560 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.300 |
| Rwork | 0.245 |
| R-free | 0.31500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gtu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.279 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.165 | 0.570 |
| Number of reflections | 30754 | |
| Completeness [%] | 76.2 | 68.4 |
| Redundancy | 2.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.6 | 22% PEG4000, 0.1M NA CITRATE,0.2M AMMONIUM ACETATE, PH5.6 |
