4GSL

Crystal structure of an Atg7-Atg3 crosslinked complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	281.556, 125.083, 71.079
Unit cell angles	90.00, 103.32, 90.00

Refinement procedure

Resolution	37.073 - 2.703
R-factor	0.1943
Rwork	0.193
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3T7E 3t7f AND 2DYT
RMSD bond length	0.009
RMSD bond angle	1.214
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.079	0.050	0.556
Number of reflections	64845
<I/σ(I)>	17.8
Completeness [%]	99.5	99.9	99
Redundancy	4.1	3.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	294	100 mM sodium citrate, pH 5.8, 13.5% PEG4000, 10% isopropanol, 5% dioxane, 88 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 294K