4GPU

Crystal structure of K. lactis Dxo1 (YDR370C) in complex with manganese

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 61 2 2
Unit cell lengths	82.130, 82.130, 260.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.850 - 2.800
R-factor	0.202
Rwork	0.202
R-free	0.25700
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4gps
RMSD bond length	0.012
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.800
Number of reflections	13599
Completeness [%]	99.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2M Sodium citrate tribasic, 23.5% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K