4GOS

Crystal structure of human B7-H4 IgV-like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-08
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0750, 1.5402
Spacegroup name	P 43 21 2
Unit cell lengths	46.500, 46.500, 115.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.910 - 1.590
R-factor	0.175
Rwork	0.174
R-free	0.19160
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.474
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHELX
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.149	38.590	1.680
High resolution limit [Å]	1.590	5.030	1.590
Total number of observations		8555	34525
Number of reflections	16143
<I/σ(I)>	17.7	13.9	1.6
Completeness [%]	90.1	98.9	100
Redundancy	13.3	12.7	13.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	200 mM tri-Potassium citrate, 2.2 M ammonium sulfate, pH 7.0, VAPOR DIFFUSION, temperature 291K
2	VAPOR DIFFUSION	7	291	200 mM tri-Potassium citrate, 2.2 M ammonium sulfate, I3C (magic triangle) soak, pH 7.0, VAPOR DIFFUSION, temperature 291K