4GKN
A2-MHC Complex carrying FATGIGIITV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97630 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.444, 46.590, 116.624 |
| Unit cell angles | 90.00, 121.53, 90.00 |
Refinement procedure
| Resolution | 99.400 - 2.753 |
| R-factor | 0.2113 |
| Rwork | 0.206 |
| R-free | 0.29950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hg1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.698 |
| Data reduction software | xia2 |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.404 | 99.404 | 2.820 |
| High resolution limit [Å] | 2.753 | 12.310 | 2.753 |
| Rmerge | 0.060 | 0.597 | |
| Total number of observations | 1086 | 6938 | |
| Number of reflections | 24462 | ||
| <I/σ(I)> | 6.9 | 10.1 | 1.3 |
| Completeness [%] | 99.7 | 98.8 | 99.9 |
| Redundancy | 3.9 | 3.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 25% PEG 8000, 200 mM Ammonium Sulphate, 200 mM MES Buffer, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
