4GK4

Human EphA3 Kinase domain in complex with ligand 90

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	53.067, 38.182, 75.841
Unit cell angles	90.00, 101.49, 90.00

Refinement procedure

Resolution	30.777 - 2.100
R-factor	0.1762
Rwork	0.173
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gsf
RMSD bond length	0.007
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.180	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.086	0.334
Number of reflections	17532
<I/σ(I)>	10.6	3.9
Completeness [%]	98.8	93.4
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1M sodium cacodylate pH 6.5, 0.15M ammonium sulf ate, 22.5% PEG 3350 , VAPOR DIFFUSION, HANGING DROP, temperature 293K