4GIL
Crystal Structure of Pseudouridine Monophosphate Glycosidase/Linear Pseudouridine 5'-Phosphate Adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.472, 77.116, 200.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.069 - 2.539 |
| R-factor | 0.1924 |
| Rwork | 0.189 |
| R-free | 0.26030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.630 |
| High resolution limit [Å] | 2.539 | 5.470 | 2.539 |
| Rmerge | 0.081 | 0.040 | 0.478 |
| Number of reflections | 32156 | ||
| <I/σ(I)> | 10.6 | ||
| Completeness [%] | 99.3 | 99.8 | 97.9 |
| Redundancy | 3.9 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG4000, Tris, sodium chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
