4GHA
Crystal structure of Marburg virus VP35 RNA binding domain bound to 12-bp dsRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-05-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.593, 99.191, 81.801 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 81.800 - 2.500 |
| R-factor | 0.21073 |
| Rwork | 0.209 |
| R-free | 0.24987 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gh9 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.800 | 2.566 |
| High resolution limit [Å] | 2.500 | 2.501 |
| Rmerge | 0.131 | 0.362 |
| Number of reflections | 20261 | |
| <I/σ(I)> | 8.5 | 2.2 |
| Completeness [%] | 100 | |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M Bis-Tris, 2% v/v Tacsimate, 18% PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
