4GHA

Crystal structure of Marburg virus VP35 RNA binding domain bound to 12-bp dsRNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, C, E, G)	Polymerase cofactor VP35	polymer	146	16302.6	4	UniProt (P35259) Pfam (PF02097)	Marburg virus - Musoke Kenya 1980 (MARV)
2	E, F (I, J)	RNA (5'-R(*CP*UP*AP*GP*AP*CP*GP*UP*CP*UP*AP*G)-3')	polymer	12	3812.3	2
3	G, H, I, J, K... (A, C, E, G, I...)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A, C, E, G: 204 - 329 (UniProt: P35259)

PDB	External Database	Details
Met 184	-	expression tag
Ala 185	-	expression tag
His 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
Val 192	-	expression tag
Asp 193	-	expression tag
Asp 194	-	expression tag
Asp 195	-	expression tag
Asp 196	-	expression tag
Lys 197	-	expression tag
Glu 198	-	expression tag
Asn 199	-	expression tag
Leu 200	-	expression tag
Tyr 201	-	expression tag
Phe 202	-	expression tag
Gln 203	-	expression tag
Cys 296	Ser 296	variant

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	72835.1
All*	Total formula weight	72835.1

*Water molecules are not included.