4GGK
Crystal structure of Zucchini from mouse (mZuc / PLD6 / MitoPLD) bound to tungstate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2716, 1.2983 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 38.859, 38.859, 213.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.270 - 2.100 |
| R-factor | 0.21144 |
| Rwork | 0.209 |
| R-free | 0.25123 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.382 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 10188 | |
| <I/σ(I)> | 18.3 | 5.6 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 50 mM Bis-Tris, pH 6.5, 18% PEG-3350, 2% tascimate, pH 6.0, 1:100 m/m ratio protein:chymotrypsin, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
