4GGD
Structural analysis of human Cdc20 supports multisite degron recognition by APC/C.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-17 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97937 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.826, 88.129, 118.351 |
| Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
| Resolution | 29.168 - 2.435 |
| R-factor | 0.1565 |
| Rwork | 0.154 |
| R-free | 0.20120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.417 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1062) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.600 | 2.490 |
| High resolution limit [Å] | 2.435 | 2.450 |
| Rmerge | 0.104 | |
| Number of reflections | 36910 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 98.4 | 93.4 |
| Redundancy | 4.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting-drop vapor diffusion | 9.3 | 293 | 50 mM CAPSO, 5% (w/v) PEG 6000, and 15.5 % MPD, pH 9.3, sitting-drop vapor diffusion, temperature 293K |
