4GEH
Crystal structure of MST4 dimerization domain complex with PDCD10
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-01-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97930, 0.97907 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.243, 84.018, 109.679 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.970 - 1.950 |
R-factor | 0.2217 |
Rwork | 0.220 |
R-free | 0.25320 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.248 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.114 | 0.971 |
Number of reflections | 52288 | |
<I/σ(I)> | 2.3 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.2 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 2% v/v Tacsimate pH 6.0, 0.1M BIS-TRIS pH 6.5, 18% w/v PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 289K |