4GAD
Crystal Structure of D230A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-07-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 80.600, 97.330, 127.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.350 |
| R-factor | 0.22282 |
| Rwork | 0.220 |
| R-free | 0.26888 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g9o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.780 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.073 | 0.482 |
| Number of reflections | 21227 | |
| <I/σ(I)> | 3.3 | |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Under Oil, Microbatch Method | 7.5 | 293 | 0.1M HEPES pH 7.5, 0.02M MgCl2 hexahydrate, 30% (w/v) D-glucose, 22% (w/v) polyacrylic acid, Under Oil, Microbatch Method, temperature 293K |
