4G7Y

Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (R217E, 2.8 A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-27
Detector	Mar 300 CCD
Spacegroup name	P 65 2 2
Unit cell lengths	121.010, 121.010, 229.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	104.800 - 2.800
R-factor	0.20887
Rwork	0.206
R-free	0.26078
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.634
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	104.800
High resolution limit [Å]	2.800
Number of reflections	24429
Completeness [%]	100.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine, 0.3 M MgCl2, 24% PEG 2000, 10% Glycerol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine, 0.3 M MgCl2, 24% PEG 2000, 10% Glycerol, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K